Terminal lipophilization of a unique DNA dodecamer by various nucleolipid headgroups: Their incorporation into artificial lipid bilayers and hydrodynamic properties

• Emma Werz and
• Helmut Rosemeyer

Beilstein J. Org. Chem. 2015, 11, 913–929, doi:10.3762/bjoc.11.103

• determination of the bilayer brightness using fluorescence microscopy [8]. In the following, we describe the insertion of six unique single-stranded DNA dodecamers carrying different nucleolipid head groups as well as cyanine-5 (Cy5) at the 5’-(n−1) position in artificial lipid bilayers. In this case we varied

• , τD (ms), of LON 10 in the presence of a lipid bilayer. Position: 1 - buffer; 2 - solution in close proximity to the bilayer; 3 - bilayer-bound. Experimental results of the incorporation of the LONs into artificial lipid bilayers with respect to the log P values of the nucleolipid head groups

• lipid bilayers, which create two compartments (cis/trans channel) of an optically transparent microfluidic sample carrier with perfusion capabilities, as shown in Figure 4. Both the cis and the trans channel were filled with saline buffered solution. The injection of the different oligonucleotides into

Specific DNA duplex formation at an artificial lipid bilayer: fluorescence microscopy after Sybr Green I staining

• Emma Werz and
• Helmut Rosemeyer

Beilstein J. Org. Chem. 2014, 10, 2307–2321, doi:10.3762/bjoc.10.240

• complexes consisting of various DNA duplexes and the fluorescent dye were studied with respect to the kinetics of their formation as well as to their stability against perfusion. Keywords: artificial lipid bilayers; lipo-oligonucleotide duplexes; nucleic acids; Sybr Green I; Introduction The post

• . Schematic illustrations (experiments A–F) of the specific DNA duplex formation at artificial lipid bilayers forming structures of various membrane-bound nucleic acid–dye complexes. A) Covalent bound Cy5-fluorophore and intercalated SG with DNA duplex were irradiated simultaneously at 635 nm and 470 nm. B